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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Dev Biol. 2021 May 20;477:164–176. doi: 10.1016/j.ydbio.2021.05.006

Figure 5. Lztfl1 knockout affects in vitro fertilization (IVF) rate.

Figure 5.

For in vitro fertilization, sperm samples were collected for the Lztfl1 KO and wild type males (2 to 6 months of age, n=3) as described above and used to fertilize oocytes collected from the oviduct of hormonally stimulated wild type C57BL/6NCr females. The fertilized oocytes were surgically transferred into the oviducts of pseudo pregnant recipient females at 2-cell stage, approximately 24 hours post-IVF. The KO mouse sperm showed a reduction in the rate of IVF (A) but not live birth (B), compared with control mice sperm. *p < 0.05.