Table 1: List of putative IFT27 binding proteins selected under stringent conditions.
The full-length IFT27 coding sequence was cloned into the Nde/BamH1 sites of pGBKT7, which was used to screen a Mate & Plate™ Library-Universal Mouse (Normalized) (Clontech, Cat#: 630482) according to the manufacturer’s instructions. The yeasts were grown on plates lacking four amino acids (Ade-Leu-His-Trp). LZTFL1 was found to be one of the putative IFT27 binding proteins. The screen also identified IFT25, which has been shown to be an IFT27 binding partner.
| Name | Ncbi number | Frequency |
|---|---|---|
| Mus musculus ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 2, mRNA | NM_009727.3 | 27 |
| Mus musculus COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) (Cops5), transcript variant 1, mRNA | NM_013715.2 | 8 |
| Mus musculus BRO1 domain and CAAX motif containing (Brox), mRNA | NM_027861.2 | 8 |
| Mus musculus signal peptide peptidase like 2A (Sppl2a), mRNA | NM_023220.2 | 7 |
| Mus musculus syndecan binding protein (Sdcbp), transcript variant 2, mRNA | NM_016807.2 | 6 |
| Mus musculus strain mixed chromosome 16, alternate assembly Mm_Celera | AC_000038.1 | 6 |
| Mus musculus solute carrier family 34 (sodium phosphate), member 2 (Slc34a2), transcript variant X1, mRNA | XM_006503807.1 | 5 |
| Mus musculus amyloid beta (A4) precursor protein-binding, family B, member 2 (Apbb2), transcript variant X12, mRNA | XM_006503697.2 | 5 |
| Mus musculus strain mixed chromosome 9, alternate assembly Mm_Celera | AC_000031.1 | 5 |
| Mus musculus nuclear receptor coactivator 7 (Ncoa7), transcript variant X4, mRNA | XM_011243155.1 | 4 |
| Mus musculus lymphocyte cytosolic protein 1 (Lcp1), transcript variant X6, mRNA | XM_006518703.2 | 4 |
| Mus musculus Yip1 domain family, member 4 (Yipf4), mRNA | NM_026417.4 | 4 |
| Mus musculus ATPase, H+ transporting, lysosomal V1 subunit D (Atp6v1d), mRNA | NM_023721.2 | 4 |
| Mus musculus heat shock protein 90, alpha (cytosolic), class A member 1 (Hsp90aa1), mRNA | NM_010480.5 | 4 |
| Mus musculus metal response element binding transcription factor 2 (Mtf2), transcript variant X10, misc_RNA | XR_389262.2 | 4 |
| Mus musculus mitofusin 2 (Mfn2), transcript variant X1, mRNA | XM_006538611.2 | 4 |
| Mus musculus annexin A2 (Anxa2), mRNA | NM_007585.3 | 4 |
| Mus musculus integrin alpha 6 (Itga6), transcript variant X3, mRNA | XM_011239310.1 | 3 |
| Mus musculus synaptotagmin IV (Syt4), mRNA | NM_009308.3 | 3 |
| Mus musculus echinoderm microtubule associated protein like 1 (Eml1), transcript variant 3, mRNA | NM_001286346.1 | 3 |
| Mus musculus homeodomain interacting protein kinase 3 (Hipk3), transcript variant 2, mRNA | NM_001145824.1 | 3 |
| Mus musculus myosin VA (Myo5a), transcript variant X7, mRNA | XM_006510834.2 | 3 |
| Mus musculus predicted gene 5092 (Gm5092), mRNA | XM_011251591.1 | 2 |
| Mus musculus C2 calcium-dependent domain containing 2 (C2cd2), transcript variant X4, mRNA | XM_011246111.1 | 2 |
| Mus musculus zinc finger protein 654 (Zfp654), transcript variant X4, mRNA | XM_011246019.1 | 2 |
| Mus musculus metadherin (Mtdh), transcript variant X6, mRNA | XM_006520153.2 | 2 |
| Mus musculus glycosyltransferase 8 domain containing 1 (Glt8d1), transcript variant X3, mRNA | XM_006519673.2 | 2 |
| Mus musculus death associated protein kinase 1 (Dapk1), transcript variant X2, mRNA | XM_006517371.2 | 2 |
| Mus musculus plastin 1 (I-isoform) (Pls1), transcript variant X1, mRNA | XM_006510746.2 | 2 |
| Mus musculus hook homolog 1 (Drosophila) (Hook1), transcript variant X3, mRNA | XM_006503487.2 | 2 |
| Mus musculus angel homolog 2 (Drosophila) (Angel2), transcript variant X4, mRNA | XM_006497186.2 | 2 |
| Mus musculus transmembrane protein 186 (Tmem186), mRNA | NM_025708.4 | 2 |
| Mus musculus Wolfram syndrome 1 homolog (human) (Wfs1), mRNA | NM_011716.2 | 2 |
| Mus musculus strain mixed chromosome 8, alternate assembly Mm_Celera | AC_000030.1 | 2 |
| Mus musculus strain mixed chromosome 2, alternate assembly Mm_Celera | AC_000024.1 | 2 |
| Mus musculus strain mixed chromosome 1, alternate assembly Mm_Celera | AC_000023.1 | 2 |
| Mus musculus calpain 7 (Capn7), transcript variant X2, misc_RNA | XR_874288.1 | 1 |
| Mus musculus predicted gene, 32444 (Gm32444), ncRNA | XR_867579.1 | 1 |
| Mus musculus TAR DNA binding protein (Tardbp), transcript variant X6, misc_RNA | XR_389859.2 | 1 |
| Mus musculus predicted gene, 35465 (Gm35465), transcript variant X2, ncRNA | XR_387598.2 | 1 |
| Mus musculus O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (Ogt), transcript variant X2, mRNA | XM_011247503.1 | 1 |
| Mus musculus IWS1 homolog (S. cerevisiae) (Iws1), transcript variant X3, mRNA | XM_011247009.1 | 1 |
| Mus musculus coiled-coil domain containing 68 (Ccdc68), transcript variant X7, mRNA | XM_011246972.1 | 1 |
| Mus musculus leucine rich repeat containing 9 (Lrrc9), transcript variant X5, mRNA | XM_011244212.1 | 1 |
| Mus musculus signal-induced proliferation-associated 1 like 1 (Sipa1l1), transcript variant X14, mRNA | XM_011244072.1 | 1 |
| Mus musculus androglobin (Adgb), transcript variant X1, mRNA | XM_011243160.1 | 1 |
| Mus musculus SET and MYND domain containing 2 (Smyd2), transcript variant X4, mRNA | XM_011238912.1 | 1 |
| Mus musculus membrane-spanning 4-domains, subfamily A, member 14 (Ms4a14), transcript variant X4, mRNA | XM_006543452.2 | 1 |
| Mus musculus predicted gene 2511 (Gm2511), transcript variant X2, mRNA | XM_006541333.1 | 1 |
| Mus musculus protein tyrosine phosphatase, non-receptor type 3 (Ptpn3), transcript variant X5, mRNA | XM_006538101.2 | 1 |
| Mus musculus tryptophan rich basic protein (Wrb), transcript variant X1, mRNA | XM_006536895.2 | 1 |
| Mus musculus cyclin G associated kinase (Gak), transcript variant X3, mRNA | XM_006534907.2 | 1 |
| Mus musculus interleukin 31 (Il31), transcript variant X1, mRNA | XM_006530498.1 | 1 |
| Mus musculus protein kinase inhibitor, alpha (Pkia), transcript variant X1, mRNA | XM_006530056.1 | 1 |
| Mus musculus Nik related kinase (Nrk), transcript variant X1, mRNA | XM_006528550.2 | 1 |
| Mus musculus GATA binding protein 6 (Gata6), transcript variant X3, mRNA | XM_006525657.2 | 1 |
| Mus musculus dynein, axonemal, heavy chain 8 (Dnah8), transcript variant X4, mRNA | XM_006523592.2 | 1 |
| Mus musculus RIKEN cDNA 2900011O08 gene (2900011O08Rik), transcript variant X1, mRNA | XM_006522482.2 | 1 |
| Mus musculus Fgfr1op N-terminal like (Fopnl), transcript variant X1, mRNA | XM_006522450.2 | 1 |
| Mus musculus zinc finger RNA binding protein (Zfr), transcript variant X3, mRNA | XM_006520059.2 | 1 |
| Mus musculus sarcolemma associated protein (Slmap), transcript variant X15, mRNA | XM_006519738.2 | 1 |
| Mus musculus THO complex 7 homolog (Drosophila) (Thoc7), transcript variant X10, mRNA | XM_006518078.2 | 1 |
| Mus musculus Rho-related BTB domain containing 3 (Rhobtb3), transcript variant X5, mRNA | XM_006517412.2 | 1 |
| Mus musculus jumonji, AT rich interactive domain 2 (Jarid2), transcript variant X5, mRNA | XM_006516860.2 | 1 |
| Mus musculus La ribonucleoprotein domain family, member 4B (Larp4b), transcript variant X5, mRNA | XM_006516478.2 | 1 |
| Mus musculus catsper channel auxiliary subunit beta (Catsperb), transcript variant X2, mRNA | XM_006515930.1 | 1 |
| Mus musculus HCLS1 binding protein 3 (Hs1bp3), transcript variant X1, mRNA | XM_006515161.2 | 1 |
| Mus musculus ubiquitin-conjugating enzyme E2N (Ube2n), transcript variant X2, mRNA | XM_006514344.2 | 1 |
| Mus musculus ankyrin repeat domain 24 (Ankrd24), transcript variant X24, mRNA | XM_006514107.2 | 1 |
| Mus musculus transmembrane and coiled coil domains 3 (Tmcc3), transcript variant X6, mRNA | XM_006513754.2 | 1 |
| Mus musculus leucine zipper transcription factor-like 1 (Lztfl1), transcript variant X2, mRNA | XM_006512403.2 | 1 |
| Mus musculus kinesin family member 9 (Kif9), transcript variant X5, mRNA | XM_006511945.2 | 1 |
| Mus musculus unc-13 homolog C (C. elegans) (Unc13c), transcript variant X7, mRNA | XM_006510934.2 | 1 |
| Mus musculus zinc finger CCCH type containing 12C (Zc3h12c), transcript variant X3, mRNA | XM_006510335.2 | 1 |
| Mus musculus zinc finger protein 426 (Zfp426), transcript variant X14, mRNA | XM_006510182.2 | 1 |
| Mus musculus adenosine monophosphate deaminase 3 (Ampd3), transcript variant X6, mRNA | XM_006507222.2 | 1 |
| Mus musculus FGFR1 oncogene partner 2 (Fgfr1op2), transcript variant X1, mRNA | XM_006507094.1 | 1 |
| Mus musculus acyl-Coenzyme A oxidase 3, pristanoyl (Acox3), transcript variant X4, mRNA | XM_006504198.2 | 1 |
| Mus musculus cytoplasmic polyadenylation element binding protein 2 (Cpeb2), transcript variant X6, mRNA | XM_006503932.2 | 1 |
| Mus musculus heat shock protein family B (small), member 11 (Hspb11, Ift25), transcript variant X2, mRNA | XM_006503425.1 | 1 |
| Mus musculus zinc finger, CCHC domain containing 11 (Zcchc11), transcript variant X1, mRNA | XM_006502978.2 | 1 |
| Mus musculus glutathione S-transferase, mu 1 (Gstm1), transcript variant X1, mRNA | XM_006501017.2 | 1 |
| Mus musculus gelsolin (Gsn), transcript variant X3, mRNA | XM_006497992.2 | 1 |
| Mus musculus abl-interactor 1 (Abi1), transcript variant X3, mRNA | XM_006497619.1 | 1 |
| Mus musculus PHD finger protein 3 (Phf3), transcript variant X7, mRNA | XM_006495840.2 | 1 |
| Mus musculus predicted gene 6644 (Gm6644), non-coding RNA | NR_037965.1 | 1 |
| Mus musculus RIKEN cDNA C130080G10 gene (C130080G10Rik), long non-coding RNA | NR_028422.1 | 1 |
| Mus musculus RIKEN cDNA A230072C01 gene (A230072C01Rik), transcript variant 2, long non-coding RNA | NR_027445.1 | 1 |
| Mus musculus thioredoxin-related transmembrane protein 3 (Tmx3), mRNA | NM_198295.2 | 1 |
| Mus musculus MORN repeat containing 2 (Morn2), mRNA | NM_194269.2 | 1 |
| Mus musculus zinc finger, MIZ-type containing 1 (Zmiz1), mRNA | NM_183208.3 | 1 |
| Mus musculus Son DNA binding protein (Son), transcript variant 1, mRNA | NM_178880.4 | 1 |
| Mus musculus ankyrin repeat and KH domain containing 1 (Ankhd1), mRNA | NM_175375.3 | 1 |
| Mus musculus eukaryotic translation initiation factor 5 (Eif5), transcript variant 1, mRNA | NM_173363.5 | 1 |
| Mus musculus SNAP-associated protein (Snapin), mRNA | NM_133854.3 | 1 |
| Mus musculus hook homolog 1 (Drosophila) (Hook1), mRNA | NM_030014.2 | 1 |
| Mus musculus zinc finger, CCHC domain containing 13 (Zcchc13), mRNA | NM_029158.2 | 1 |
| Mus musculus small nuclear ribonucleoprotein polypeptide G (Snrpg), mRNA | NM_026506.2 | 1 |
| Mus musculus integrin beta 3 binding protein (beta3-endonexin) (Itgb3bp), mRNA | NM_026348.3 | 1 |
| Mus musculus plexin domain containing 2 (Plxdc2), mRNA | NM_026162.6 | 1 |
| Mus musculus cytochrome b5 type B (Cyb5b), mRNA | NM_025558.5 | 1 |
| Mus musculus RAS related protein 1b (Rap1b), mRNA | NM_024457.2 | 1 |
| Mus musculus charged multivesicular body protein 1B (Chmp1b), mRNA | NM_024190.2 | 1 |
| Mus musculus solute carrier family 6 (neurotransmitter transporter), member 14 (Slc6a14), mRNA | NM_020049.4 | 1 |
| Mus musculus phosphatidylethanolamine binding protein 1 (Pebp1), mRNA | NM_018858.2 | 1 |
| Mus musculus sulfotransferase family 1D, member 1 (Sult1d1), mRNA | NM_016771.3 | 1 |
| Mus musculus anterior gradient 2 (Agr2), mRNA | NM_011783.2 | 1 |
| Mus musculus solute carrier family 34 (sodium phosphate), member 2 (Slc34a2), mRNA | NM_011402.3 | 1 |
| Mus musculus 6-pyruvoyl-tetrahydropterin synthase (Pts), mRNA | NM_011220.2 | 1 |
| Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), mRNA | NM_011055.2 | 1 |
| Mus musculus eukaryotic translation initiation factor 3, subunit A (Eif3a), mRNA | NM_010123.3 | 1 |
| Mus musculus CD24a antigen (Cd24a), mRNA | NM_009846.2 | 1 |
| Mus musculus neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8), mRNA | NM_008683.3 | 1 |
| Mus musculus inhibitor of growth family, member 1 (Ing1), transcript variant 2, mRNA | NM_001302451.1 | 1 |
| Mus musculus neuron specific gene family member 2 (Nsg2), transcript variant 3, mRNA | NM_001290681.1 | 1 |
| Mus musculus prion protein (Prnp), transcript variant 2, mRNA | NM_001278256.1 | 1 |
| Mus musculus tetraspanin 2 (Tspan2), transcript variant 2, mRNA | NM_001243132.1 | 1 |
| Mus musculus doublecortin-like kinase 1 (Dclk1), transcript variant 7, mRNA | NM_001195540.1 | 1 |
| Mus musculus remodeling and spacing factor 1 (Rsf1), mRNA | NM_001081267.2 | 1 |
| Mus musculus RNA binding protein gene with multiple splicing (Rbpms), transcript variant 2, mRNA | NM_001042674.2 | 1 |
| Mus musculus mitochondrion, complete genome | NC_005089.1 | 1 |
| Mus musculus strain C57BL/6J chromosome 11, GRCm38.p3 C57BL/6J | NC_000077.6 | 1 |
| Mus musculus strain C57BL/6J chromosome 9, GRCm38.p3 C57BL/6J | NC_000075.6 | 1 |
| Mus musculus strain mixed chromosome 17, alternate assembly Mm_Celera | AC_000039.1 | 1 |
| Mus musculus strain mixed chromosome 15, alternate assembly Mm_Celera | AC_000037.1 | 1 |
| Mus musculus strain mixed chromosome 13, alternate assembly Mm_Celera | AC_000035.1 | 1 |
| Mus musculus strain mixed chromosome 7, alternate assembly Mm_Celera | AC_000029.1 | 1 |
| Mus musculus glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant X8, misc_RNA | XR_375489.2 | 1 |
| Mus musculus aldehyde dehydrogenase 4 family, member A1 (Aldh4a1), transcript variant X1, mRNA | XM_011250224.1 | 1 |
| Mus musculus metadherin (Mtdh), transcript variant X6, mRNA | XM_006520153.2 | 1 |
| Mus musculus oxysterol binding protein-like 8 (Osbpl8), transcript variant X3, mRNA | XM_006513638.2 | 1 |
| Mus musculus CCR4-NOT transcription complex, subunit 7 (Cnot7), transcript variant X8, mRNA | XM_006509307.2 | 1 |
| Mus musculus interferon induced transmembrane protein 3 (Ifitm3), mRNA | NM_025378.2 | 1 |
| Mus musculus Shc SH2-domain binding protein 1-like (Shcbp1l), mRNA | NM_001033162.2 | 1 |