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. Author manuscript; available in PMC: 2022 Jul 13.
Published in final edited form as: Lab Chip. 2021 Jul 13;21(14):2730–2737. doi: 10.1039/d1lc00293g

Figure 2.

Figure 2.

Detection of SARS-CoV-2 by RT-RPA/CRISPR-Cas12a assay in reaction tubes. A. The target genes and detection strategy of SARS-CoV-2. B. Fluorescence detection of N and S genes of SARS-CoV-2 by RT-RPA/CRISPR-Cas12a assay. NC, negative control without SARS-Cov-2 RNA. Tubes 1-4 contain, respectively, 1, 10, 102, 103 copies SARS-CoV-2 RNA spiked in the reaction solution. The images were taken under the LED blue transilluminator and ChemiDocTM MP Imaging System, respectively. C. Threshold time of the N gene detection of SARS-CoV-2 at different concentrations (0, 1, 10, 102, 103 copies). D. Threshold time of the S gene detection of SARS-CoV-2 at different concentrations (0, 1, 10, 102, 103 copies). *** indicate a significant difference of the NC and 1 copy with 10 to 103 copies SARS-CoV-2 in the reaction solution (p < 0.001, t-test). Error bars denote s.d. (n =3).