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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Dev Biol. 2021 Jun 6;477:251–261. doi: 10.1016/j.ydbio.2021.06.003

Figure 1: BMP signaling activity peaks during midbrain neural crest delamination.

Figure 1:

(A) Schematic diagram representing the BMP-sensitive reporter construct used in this study. BMP-responsive elements (BRE) drive expression of histone H2B tagged with GFP harboring the destabilization domain from mouse ornithine decarboxylase (d2). (B) Schematic diagrams illustrating neural crest development in specified (6ss), delaminating (8ss), and migrating (10ss) neural crest cells at the midbrain axial level. (C) Gastrulating chicken embryos were electroporated with DNA constructs that ubiquitously express RFP (cyan) and BRE::H2B-d2EGFP (pseudocolored based on color scale on right), and examined at 6ss (C1), 8ss (C2), and 10ss (C3). Transverse sections were immunolabeled to label PAX7 expression in neural crest cells (magenta). Scale bar represents 50 μm. (D) BRE activity was measured within each PAX7-positive neural crest nucleus and normalized to RFP as an electroporation control. *** p<0.001, n.s. not significant, Kruskal-Wallis one-way analysis with Bonferroni-adjusted Dunn’s post-hoc analysis. ss, somite stage; pNC, premigratory neural crest; mNC, migratory neural crest.