CHD7 stained paraffin sections from E14.5 (A, D, G), E16.5 (B, E, H), and E18.5 (C, F, I) Chd7+/+ embryos show CHD7+ cells present in the spiral ganglion (white dashed outlines) at E14.5 and E16.5, but minimal CHD7+ cells at E18.5. CHD7+ cells are also present in the cochlear epithelium (CE in A, B, C, G, H, I) at these stages. Neurofilament staining of the same sections (D-F) identifies spiral ganglion neurons at each stage. Scale bars = 50 μm.