Anti-CHD7 staining and Zsgreen reporter fluorescence overlap in the spiral ganglion of E15.5 of Shh+/Cre;Chd7+/flox;Zsgreen (A-C) mice. CHD7 is reduced in the ganglion of E15.5 Shh+/Cre;Chd7flox/flox;Zsgreen (D-F) ears. Whole-mount cochleae from P1 Chd7+/flox and Shh+/Cre;Chd7flox/flox mice stained with anti-Neurofilament (H, I, K, L) and anti-Myo7A (G, I, J, L) shows normal appearing hair cells and neurites. Scale bars A-F = 50 μm; G-L = 45 μm.