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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Neurotox Res. 2021 Apr 26;39(4):1227–1237. doi: 10.1007/s12640-021-00366-6

Figure 1:

Figure 1:

Paraquat preferentially destroys outer hair cells (OHCs), inner hair cells (IHCs) and nerve fibers (NFs) in the base of the rat cochlea. Representative confocal images of cochlear surface preparations cultured for 24 h and then labeled with Alexa Fluor 488 conjugated phalloidin (green) that labels the stereocilia and cuticular plate of OHCs and IHCs and an antibody against neurofilament 200 conjugated to a secondary antibody labeled with Alexa Fluor 555 (red) that binds to NFs. Photomicrographs of Control (0 μM paraquat) cochlear surface preparations from the apex (A) and base (B) of the rat cochlea showing three parallel rows OHCs, a single row of IHCs and fascicles of NFs projecting out radially to the IHCs and OHCs. After 24 h treatment with 50 μM paraquat, OHCs, IHCs and NFs (arrows) were largely intact in the apex of the cochlea (C) whereas many OHCs and IHCs (arrowhead) were shrunken or missing in the base of the cochlea and many NFs were missing (dashed arrow).