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. 2021 Jul 13;12:4290. doi: 10.1038/s41467-021-24473-2

Fig. 5. IL-15 potentiates IEL cytotoxicity.

Fig. 5

a Estimated protein copy number/cell of cytotoxic molecules granzyme A (GzmA), granzyme B (GzmB), Perforin, Munc13-4, STXBP2 and RAB27A. Data are shown for all IEL subsets ± 24 h IL-15/Rα stimulation (n = 4 biologically independent samples), statistical significance was derived from two-tailed empirical Bayes moderated t-statistics performed in limma on total proteome, see Supplementary Data 3. b Flow cytometric analyses of intracellular GzmA and GzmB in freshly isolated IEL compared to those cultured with 100 ng/mL IL-15/Rα for 24 h. Data are presented as mean fluorescence intensity (MFI) and percentage positive cells for GzmB (gating strategy shown in Supplementary Fig. 6c), (n = 3 biologically independent experiments), analysed by two-way ANOVA with Sidak’s multiple comparisons test. c Luciferase-transduced K562 cells were co-cultured for 24 h with freshly isolated IEL at an effector to target (E:T) ratio of 40:1. Cells were treated with either 10 ng/mL or 100 ng/mL IL-15/Rα (±aCD3). d Luciferase-transduced K562 cells were co-cultured for 24 h with freshly isolated IEL or WT and GzmA/B dKO IEL that had been pre-treated with 100 ng/mL IL-15/Rα (±aCD3) for 72 h, at an E:T ratio of 40:1. c, d Bar graphs represent the percentage of specific lysis for each condition, (n = 3 biologically independent experiments) data were analysed by one-way ANOVA, with Sidak’s multiple comparisons test. All error bars are mean ± s.e.m.