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. 2021 Jul 13;12:4278. doi: 10.1038/s41467-021-24617-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Nucleotide position of the six additional ORFs and the six known ORFs in the TYLCV genome, size (in aa) and predicted molecular weight (MW; in kDa) of the corresponding encoded proteins, and domains or signals predicted in the protein sequence. TM: transmembrane domain; NLS: nuclear localization signal; cTP: chloroplast transit peptide. b Subcellular localization of the proteins encoded by ORF1-6 fused to GFP at their C-terminus transiently expressed in N. benthamiana leaves. Scale bar: 25 μm. c Co-localization of ORF2-GFP with the ER marker RFP-HDEL. Scale bar: 25 μm. d Co-localization of ORF3-GFP with the mitochondrial stain MitoTracker Red. Scale bar: 25 μm. e Co-localization of ORF4-GFP with the ER marker RFP-HDEL and the cis-Golgi marker SYP32-RFP. Scale bar: 10 μm. f Co-localization of ORF5-GFP with the cis-Golgi marker SYP32-RFP. Scale bar: 25 μm. g Co-localization of ORF6-GFP with the cis-Golgi marker SYP32-RFP. Scale bar: 25 μm. These experiments were repeated at least three times with similar results; representative images are shown. The TYLCV isolate used in these experiments is TYLCV-Alm.