Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 13;12:4278. doi: 10.1038/s41467-021-24617-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a RT-PCR analysis of V3 transcripts from TYLCV-infected or uninfected N. benthamiana plants. M: DNA ladder marker. NC1: negative control 1 (reverse-transcription of total RNA extracted from uninfected plants with RT Primers). NC2: negative control 2 (reverse-transcription of total RNA extracted from uninfected plants with V3-specific primers). TYLCV: reverse-transcription of total RNA extracted from TYLCV-infected plants with V3-specific primers. This experiment was repeated three times with similar results; representative images are shown. b Transcriptional start site analysis of TYLCV V3 by 5ʹ RACE. TSS: transcription start site. A2058: V3 TSS captured by RSV cap-snatching (Lin et al., 2017). c Activity of pV3 promoter (and p35S promoter as positive control) in promoter-GUS fusions in transiently transformed N. benthamiana leaves at 2 dpi. EV: empty vector. This experiment was repeated three times with similar results; representative images are shown. d Quantification of relative GUS activity in samples from (c). Data are the mean of three independent biological replicates. Error bars represent SD. An asterisks indicate a statistically significant difference according to unpaired Student’s t test (two-tailed), *** p < 0.001. e Activity of pV3 promoter (and p35S promoter as positive control) in promoter-GFP fusions in transiently transformed N. benthamiana leaves at 2 dpi. Scale bar: 100 μm. EV: empty vector. This experiment was repeated three times with similar results; representative images are shown. f Quantification of relative GFP intensity in samples from (e). Data are the mean of three independent biological replicates. Error bars represent SD. Asterisks indicate a statistically significant difference according to unpaired Student’s t test (two-tailed), ** p < 0.01, *** p < 0.001. g Western blot analysis of GFP protein from (e). Ponceau S staining of the large RuBisCO subunit serves as loading control. EV: empty vector. This experiment was repeated three times with similar results; representative images are shown. h Co-localization of V3-GFP with the cis-Golgi maker SYP32-RFP (upper panel) and co-localization of V3-YFP with the ER maker mCherry-HDEL (lower panel). Scale bar: 20 μm. The TYLCV isolate used in these experiments is TYLCV-BJ. The original data from all experiments and replicates can be found in the Source data file.