. 2021 Jul 13;11:14356. doi: 10.1038/s41598-021-93688-6

Table 7.

Primer gene sequence and PCR conditions.

	Nucleotide sequence	Target Gene	PCR condition (35 cycles)			Product size (bp)	References
	Nucleotide sequence	Target Gene	Denaturing	Annealing	Extension	Product size (bp)	References
EAEF	5′-AAACAGGTGAAACTGTTGCC-3′	eaeA	95 °C, 60 s	55 °C,60 s	72 °C,60 s	450	⁸⁶
EAER	5′-CTCTGCAGATTAACCTCTGC-3′	eaeA	95 °C, 60 s	55 °C,60 s	72 °C,60 s	450	⁸⁶
EVSF	5′-ATCAGTCGTCACTCACTGGT-3′	stx1	95 °C, 60 s	55 °C,60 s	72 °C,60 s	110	⁸⁷
EVCR	5′-CTGCTGTCACAGTGACAAA-3′	stx1	95 °C, 60 s	55 °C,60 s	72 °C,60 s	110	⁸⁷
EVTF	5′-CAACACTGGATGATCTCAG-3′	stx2	95 °C, 60 s	55 °C,60 s	72 °C,60 s	350	⁸⁷
EVTR	5′-CCCCCTCAACTGCTAATA-3′	stx2	95 °C, 60 s	55 °C,60 s	72 °C,60 s	350	⁸⁷

F Forward, R Reverse, eae effacing and attaching, ev Verocytotoxin, stx shiga toxin, bp base pair.