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. 2021 Jul 13;12(7):696. doi: 10.1038/s41419-021-03991-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Differential expression proteins. The basic statistic used for significance analysis is fold change. Results include (log2) the fold changes of every protein and its contrast individually. Scatter plot: red presents upregulation, blue presents downregulation, and grey presents no difference. b Whole-cell extracts from NCI-N87 cells after treated with or without 1 μM TG for 12 h were incubated in the arrays of RTK antibody. Phosphorylated proteins were measured by incubating with anti-phosphotyrosine horseradish peroxidase. c Representative western blot and quantification for IGF-1R and FGFR1 in NCI-N87 and MKN45 cells after treated with or without 1 μM TG for 12 h. d Western blot analysis of indicated proteins in miR-301a-3p knockdown NCI-N87 and MKN45 cells treated with 1 μM TG for 12 h followed by transfection with LRIG1 siRNAs (siLRIG1) or NC. e qRT-PCR analysis of IGF-1R and FGFR1 mRNA in miR-301a-3p knockdown NCI-N87 and MKN45 cells after transfection with siLRIG1 or control (n = 3). f CCK8 assay of ER stressed miR-301a-3p knockdown NCI-N87 and MKN45 cells transfected with siLRIG1 or control before or control and treated with TG and followed by trastuzumab treatment (n = 3). g Western blot analysis of certain proteins in ER stressed miR-301a-3p knockdown NCI-N87 and MKN45 cells transfected with siLRIG1 or control followed by 400 μg/ml trastuzumab treatment for 72 h. h Flow cytometry was used to measure the apoptosis of ER stressed miR-301a-3p knockdown NCI-N87 and MKN45 cells transfected with siLRIG1 or control after exposure to 400 μg/ml trastuzumab for 72 h (n = 3). Statistical analysis was performed by Student’s t-test, error bars indicate SD (*p < 0.05, **p < 0.01, ***p < 0.001).