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. 2021 Jul 13;12(7):696. doi: 10.1038/s41419-021-03991-3

Fig. 6. Exosomes released by ER stress transmitted trastuzumab resistance through transporting miR-301a-3p in vitro.

Fig. 6

a qRT-PCR analysis of miR-301a-3p in the CM of NCI-N87 and MKN45 cells treated by 1 μM TG for 12 h following by treatment of RNase (2 mg/ml) only or a combination of RNase and Triton X-100 (0.1%) for 20 minutes (n = 3). b qRT-PCR analysis of miR-301a-3p in exosomes extracted from the CM of NCI-N87 and MKN45 cells treated with or without 1 μM TG for 12 h (n = 3). c qRT-PCR analysis of miR-301a-3p in NCI-N87 and MKN45 cells after the cells were incubated with certain exosomes (PBS as control) for 48 h (n = 3). d CCK8 assay of NCI-N87 and MKN45 cells treated by different sources of CM for 24 h before trastuzumab treatment for 72 h (n = 3). e CCK8 assay of NCI-N87 and MKN45 cells first incubated with indicated exosomes and transfected with miR-301a-3p inhibitors or NC before trastuzumab treatment at indicated concentrations for 72 h (n = 3). f Flow cytometry was used to measure the apoptosis of NCI-N87 and MKN45 cells incubated with indicated exosomes and transfected with miR-301a-3p inhibitors followed by 400 μg/ml trastuzumab treatment for 72 h (n = 3). g Exosomes isolated from NCI-N87 and MKN45 cells were electroporated with miR-301a-3p mimics or control. NCI-N87 and MKN45 cells were cultured with certain exosomes for 48 h and then subjected to CCK8 assay upon trastuzumab treatment at indicated concentrations for 72 h (n = 3). Statistical analysis was performed by Student’s t-test and one-way ANOVA, error bars indicate SD (**p < 0.01, ***p < 0.001).