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. 2021 Jul 13;11:14364. doi: 10.1038/s41598-021-93664-0

Figure 5.

Figure 5

Dithionite does not permeate across the membrane of pGUVs. (a) Fluorescence time trace of 10 µM Alexa Fluor 647 hydrazide in 250 mM sucrose solution upon addition of 10 mM dithionite. A buffer control (0 mM dithionite) is shown for comparison. The arrowhead indicates dithionite or buffer addition. (b) Confocal image of pGUVs with trapped Alexa Fluor 647 hydrazide. A region of interest (ROI) was placed within the GUV for fluorescence quantification, from which the mean of four background ROIs was subtracted. (c) Dot plot of Alexa Fluor 647 hydrazide fluorescence of individual pGUVs 12 min after buffer or dithionite addition (n = 84). Black lines indicate mean ± s.d. (unpaired t test with Welch’s correction, *P < 0.0001).