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. 2021 Jun 30;9:675998. doi: 10.3389/fcell.2021.675998

FIGURE 5.

FIGURE 5

6C can maintain the morphology and function of mCEC in long-term culture and subculture. (A) Morphological comparison of mCEC cultured with different compositions in long-term culture. (B) Statistical analysis of the diameters of long-term cultured cells. (C) qRT-PCR analysis of P63, Pax6, K12, ZEB1/2, and α-SMA gene expression (normalized to 6C). (D) Morphology of mCEC subculture. (E) Statistical analysis of the diameters of the cells. (F) qRT-PCR analysis of EMT-related genes, mouse corneal epithelial function-related genes and cell proliferation marker Ki67 (normalized to P0). (G) Immunofluorescence staining of EMT-related genes α-SMA (green) and ZEB1 (red). Nuclei were stained with DAPI (blue). Data expressed as the means ± SEM from three separate experiments (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significance).