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. 2021 Jun 30;9:675998. doi: 10.3389/fcell.2021.675998

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Copyright © 2021 An, Wang, Jin, Zhou, Gao, Chen, Reinach, Liu, Xue and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of 6C on mouse cornea ex vivo culture. (A) Schematic diagram of air-exposed culture of mouse cornea. (B) HE staining results of mouse cornea after air exposure culture. The epithelium in the 6C treatment group was relatively intact, while the control group had severe shedding (the white dotted line represents the edge of the corneal stroma and the red dotted line represents the boundary between the epithelial layer and the stromal layer). (C) Wholemount immunostaining of F-actin (red). Nuclei were stained with DAPI (blue). (D) Immunofluorescence staining of ZO-1 (green), P63 (red), K12 (green), K14 (red) and Pax6 (green). Nuclei were stained with DAPI (blue).