FIGURE 7.

6C helps to construct tissue engineered mouse corneal epithelium. (A) Schematic diagram of tissue engineering mouse corneal epithelial sheet culture. (a) The isolated mouse corneal epithelium was digested into single cells with trypsin, and then planted into insert. After the cells were confluent, they were cultured in air exposure. (b) Divide the isolated corneal epithelium into two and plant it on 12-well cell culture inserts. After the cells are covered with the insert, do air exposure culture. (B) When epithelial single cells were cultured to the seventh day, the cells in the 6C treatment group were almost confluent. (C) HE staining results of mCEC after air exposure culture. The cells in the 6C treatment group were stratified to two layers. (D) Immunofluorescence staining of Z0-1 (green), P63 (red), K12 (green), K14 (red) and Pax6 (green). Nuclei were stained with DAPI (blue). (E) Morphological comparison of corneal epithelial sheets cultured with corneal epithelium. In the 6C treatment group, during the culture of the epithelial sheet, the epithelial cells crawled out from the edge, and the surface cells gradually fell off. By the tenth day of culture, the cells were almost confluent, but the epithelium of the control group hardly grew. (F) HE staining results of corneal epithelium after air exposure. (G) Immunofluorescence staining of Z0-1 (green), P63 (red), K12 (green), K14 (red) and Pax6 (green). Nuclei were stained with DAPI (blue).