Figure 1.
Overview of GFP immunoprecipitation methodology. Crude thylakoid membranes from GFP-labelled PSI, PSII, Cyt b6f, ATPase and WT strains were prepared by centrifugation and were solubilized by 1% digitonin. Proteins that interact with GFP-labelled proteins are immunoprecipitated with GFP pull-down assay and subjected to mass spectrometric analysis. Triplicated experiments were conducted for each strain.
