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. 2021 Jun 15;25(14):6786–6799. doi: 10.1111/jcmm.16682

FIGURE 3.

FIGURE 3

Increased inflammation in the absence of LMP7. Immunofluorescence in frozen pancreas sections was performed using macrophages (CD68; A) and neutrophil (LY6G; B) markers antibodies. Quantification of positive cells for each leukocyte population was calculated based on the total number of cells, visualized by DAPI nuclei staining (CD206 data not shown). 0 h (n = 3‐4), 8 h and 24 h (n = 8‐9). B, MPO activity detected in lung using chromogenic substrate. 0 h (n = 12‐13), 8 h (n = 19‐23) and 24 h (n = 6‐9). C, Transcripts levels of IL‐1β, CXCL‐10 and IFN‐β genes were determined by real‐time PCR using specific primers with 5S as housekeeping. LMP7+/+ groups—0 h (n = 4‐5), 8 h and 24 h (n = 4‐7); LMP7−/− groups—0 h (n = 4), 8 h (n = 10‐13) and 24 h (n = 4‐9). D, Interleukin‐6 levels were measured in serum by CBA assay. Data were normalized to the values of the 8 h LMP7+/+ group. 0 h (n = 10‐13), 8 h and 24 h (n = 20‐25). Scale bars, 20 µm. Data are representative of five independent experiments and expressed as means ± SEM. *P < .05, **P < .01