FIGURE 1.

Hypoxia‐induced p300 interacted with XBP1s to acetylate XBP1s in RAW264.7 cells. RAW264.7 cells were assigned to normal, hypoxia, hypoxia +0.1% DMSO (vehicle) and hypoxia +L002 groups. A, Western blot was performed to detect p300, p‐IRE1α (Ser724), IRE1α and XBP1s protein levels. B, The relative protein levels of p300, p‐IRE1α and XBP1s were analysed. ^*** P <.005 vs the normal group; ^### P <.005 vs the hypoxia group. C, RAW264.7 cell lysates were immunoprecipitated with anti‐XBP1s antibody and immunoblotted with anti‐Ac‐K and anti‐p300 antibodies. D, RAW264.7 cell lysates were immunoprecipitated with anti‐p300 antibody and immunoblotted with anti‐Ac‐K and anti‐XBP1s antibodies. n = 3/group