FIGURE 2.

IH‐induced the activation of NF‐κB and the up‐regulation of proinflammatory cytokines. (A, B) The expression of NF‐κB p65 and IκB‐α in BV‐2 cells under normoxia and IH conditions was detected by Western blot analysis. n = 3 per group. ^*** P < .001 versus the normoxia group. (C) The qRT‐PCR showed the expression of NF‐κB p65 and IκB‐α in BV‐2 cells under normoxia and IH conditions. n = 3 per group. ^*** P < .001 versus the normoxia group. (D) The expression of IL‐1β and TNF‐α in BV‐2 cells under normoxia and IH conditions was detected by ELISA. n = 3 per group. ^*** P < .001 versus the normoxia group. Normoxia: BV‐2 cells were cultured in normoxia condition; IH: BV‐2 cells were exposed to IH. ELISA, enzyme‐linked immunosorbent assay; NF‐κB, nuclear factor kappa B; IL‐1β, interleukin‐1β; TNF‐α, tumour necrosis factor‐α