TABLE 1.
Primary antibodies used for immunohistochemistry (IHC) and immunofluorescence (IF)
| Primary antibody specificity | Clonality | Source | Dilution | |||||
|---|---|---|---|---|---|---|---|---|
| IHC‐FFPE | IF‐FFPE | IF‐FFOE | ||||||
| Dog | Mouse | Dog | Mouse | Dog | Mouse | |||
| CD107b | mc rat | MCA2293, Clone M3/84, Bio‐Rad Laboratories Inc, | ‐ | 1:600 | ‐ | ‐ | ‐ | 1:200 |
| CD204 | mc mouse | MAB1710, Clone SRA‐E5, Abnova Corporation | 1:500 | ‐ | 1:250 | ‐ | ‐ | ‐ |
| CD45 | mc rat | CD45 Monoclonal Antibody (30‐F11), eBioscience™, Invitrogen, Thermo Fisher Scientific | ‐ | 1:400 | ‐ | ‐ | ‐ | 1:100 |
| CNPase | mc mouse | MAB326, clone 11‐5B, Sigma‐Aldrich, Merck KGaA | 1:100 | 1:100 | 1:100 | 1:100 | ‐ | ‐ |
| GFAP | mc mouse | G3893, Sigma‐Aldrich, Merck KGaA, Darmstadt, Germany | 1:800 | 1:800 | 1:400 | 1:400 | ‐ | ‐ |
| GS | pc rabbit | PA5‐28940, Invitrogen, Thermo Fisher Scientific | 1:4000 | 1:2000 | 1:2000 | 1:2000 | ‐ | ‐ |
| Iba1 | pc goat | 011‐27991, FUJIFILM Wako Pure Chemical Corporation | ‐ | ‐ | 1:400 | 1:400 | ‐ | ‐ |
| Kir 4.1 | pc rabbit | APC‐035, Alomone laboratories Ltd, | 1:2000 | 1:4000 | 1:1000 | 1:2000 | ‐ | ‐ |
| Kir 4.1‐AG | pc rabbit | APC‐035‐AG, Alomone laboratories Ltd, | ‐ | ‐ | ‐ | ‐ | 1:200 | 1:500 |
| Nestin | pc rabbit | AP 07829PU‐N, OriGene Technologies | 1:500 | 1:500 | ‐ | ‐ | ‐ | ‐ |
| NeuN | mc mouse | MAB377, Clone A60, Sigma‐Aldrich, Merck KGaA | ‐ | ‐ | 1:800 | 1:800 | ‐ | ‐ |
| NG2 | pc rabbit | AB5320, Sigma‐Aldrich, Merck KGaA | ‐ | ‐ | ‐ | ‐ | ‐ | 1:100 |
| Periaxin | pc rabbit | HPA001868‐100UL, Sigma‐Aldrich, Merck KGaA | 1:5000 | 1:5000 | ‐ | ‐ | 1:1000 | 1:1000 |
| Sox2 | mc rabbit | 3579S, Cell Signaling Technology Inc | 1:50 | 1:50 | 1:50 | ‐ | ‐ | ‐ |
Abbreviations: CNPase, 2′,3′‐cyclic‐nucleotide 3′‐phosphodiesterase; FFOE, fresh‐frozen, OCT‐embedded; FFPE, formalin‐fixed, paraffin‐embedded; GFAP, glial fibrillary acidic protein; GS, glutamine synthetase; Iba1, ionized calcium‐binding adapter molecule 1; Kir 4.1, inwardly rectifying potassium channel 4.1; mc, monoclonal; NG2, neural/glial antigen 2; pc, polyclonal; Sox2, sex determining region Y‐box 2.