FIGURE 7.

Effects of PLAC8 on breast tumour development in vivo A. PLAC8 silencing in MCF‐7/ADMR significantly inhibited xenografts growth. Shown are representative tumour images harvested at four weeks after implantation. B, C. Changes in tumour volume and tumour weight of MCF‐7/ADMR infected with the control siRNA or PLAC8 siRNA from week 1 to week 4. Data are shown mean ± SD. D. cell viability assay was performed and knockdown of PLAC8 inhibited cell proliferation of MCF‐7/ADMR without ADM treatment in vitro. E‐G. Combined therapy of rapamycin treatment and PLAC8 knockdown led to decreased xenografts tumours size and weight, and has a synergistic effect on inhibiting tumour growth. H, I. Immunohistochemistry analysis with PLAC8 antibody and Ki67 antibody on MCF‐7/ADMR cells infected with the control siRNA or PLAC8 siRNA. Representative sections are shown. Black bars = 50 μm. J. Immunohistochemistry analysis of LC3 and p62 expression in MCF/ADR (CON) and MCF‐7/ADMR (siPLAC8) groups. K. Per cent of PLAC8+, Ki67+, LC3+ and p62+ cells were quantified. Each bar represents the mean ± SD of three independent experiments. *P < .05, **P < .01, ***P < .001