FIGURE 1.

Effect of metformin on AMPK phosphorylation (A‐B) and HUA‐inhibited glucose uptake induced by insulin in primary cardiomyocytes (C). (A‐B) Western blot analysis of phosphorylated and total AMPK levels. (C) Pre‐treated with metformin (10 μmol/L) for 60 minutes protected against HUA (15mg/dL for 24 hours) inhibited 2‐NBDG uptake induced by insulin in primary cardiomyocytes, as shown by flow cytometry analysis. (A) *P < .05 vs. 0, 15 and 30 min. (B) *P < .05 vs. 0 and 5uM. (C) *P < .05 vs. Ins+HUA. Data are mean ± SD from 4 separate experiments. Ins: insulin. HUA: high uric acid. Met: metformin. 2‐NBDG: 2‐[N‐(7‐Nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)amino]‐2‐deoxy‐d‐glucose