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5‐amino‐4‐imidazole‐1‐β‐D‐carboxamide ribofuranoside (AICAR, another AMPK activator) protected against HUA inhibited 2‐NBDG uptake induced by insulin via AMPK signal pathways in primary cardiomyocytes. (A) 2‐NBDG uptake assay detected by fluorescence microscopy. (B‐C) 2‐NBDG uptake assay detected by flow cytometry. *P < .05 vs. Ins and Ins+HUA+AICAR, #P <.01 vs. Ins+HUA+AICAR+CC. Data are mean ± SD from 4 separate experiments
