FIGURE 2.

Knockdown of MTHFD2 inhibits proliferation and promotes apoptosis in LUAD cells. A, Western blot analysis for MTHFD2 expression in NSCLC cell lines and human bronchial epithelial cells (HBECs). B and C, Western blot confirmed the knockdown efficiency of MTHFD2 in PC‐9 and H1975 cells with siRNA (#1, #2). siNC, negative control siRNA. D, CCK‐8 assay assessed the proliferation of PC‐9 and H1975 cells after transfection. E and F, The effect of MTHFD2 knockdown on cell cycle in PC‐9 and H1975 cells as indicated treatment for 48 h. G and H, Cell apoptosis (Q2 + Q4) of PC‐9 and H1975 were measured using Annexin Ⅴ‐FITC/PI staining after transfection with siNC or siMTHFD2 (si#1, si#2) for 48 h. *P < .05, **P < .01, ***P < .001