FIGURE 4.

MTHFD2 knockdown inhibits LUAD tumorigenesis and metastasis in nude mice. A, Tumour growth curves were recorded from established xenograft model of stable MTHFD2 knockdown or negative control PC‐9 cells. shMTHFD2, stable MTHFD2 knockdown. shNC, negative control. B, Photograph of xenograft model and dissected tumours. C, Tumour weight of the dissected tumours. D, E, F and G, The representative staining and scores of MTHFD2, Ki67 and TUNEL from xenograft tumours. Scale bar, 50 μm. H, Metastatic nodules in the lungs of nude mice tail vein model (three sections evaluated per lung). Scale bar, 200 μm. *P < .05, **P < .01, ***P < .001