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. 2021 Jun 13;25(14):7013–7027. doi: 10.1111/jcmm.16715

FIGURE 5.

FIGURE 5

MTHFD2 knockdown inhibits the AKT/GSK‐3β/β‐catenin signalling pathway. A, GSEA revealed high MTHFD2 expression was associated with protein serine threonine kinase activity gene set based on TCGA LUAD samples. NES, normalized enrichment score. B and C, Western blot analysis for p‐AKT (S473), AKT, p‐ERK1/2, ERK1/2 and nuclear β‐catenin expression of PC‐9 and H1975 cells after transfection. D and E, Western blot analysis for p‐AKT (S473), AKT, p‐ GSK‐3β (S9), GSK‐3β, β‐catenin and MTHFD2 expression of PC‐9 and H1975 cells after transfection with siNC or si#2 (48 h), vector or MTHFD2 plasmid (24 h) in the presence of DMSO or MK‐2206 (10 μM, 24 h) as indicated. *P < .05, **P < .01, ***P < .001