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. 2021 Jun 13;25(14):7013–7027. doi: 10.1111/jcmm.16715

FIGURE 7.

FIGURE 7

MTHFD2 overexpression is mediated by down‐regulation of miR‐30a‐3p in LUAD cells. A, The common miRNAs targeting MTHFD2 were predicted by the miRWalk, miRDB, miRanda and TargetScan Databases. B, The relative expression levels of miR‐30a‐3p in LUAD samples compared with normal lung tissues from TCGA database using starBase. C, Correlation analysis of relationship between MTHFD2 and miR‐30a‐3p mRNA expression levels in LUAD samples from TCGA database using starBase. D, RT‐qPCR analysis of miR‐30a‐3p mRNA expression levels in LUAD cell lines compared with HBECs. E, F and G, RT‐qPCR and western blot analysis for MTHFD2 mRNA and protein levels in PC‐9 and H1975 cells after transfection with NC mimics or miR‐30a‐3p mimics. H, Schematic of miR‐30a‐3p putative binding site in the wild‐type (WT) and mutant (MUT) 3′UTR of MTHFD2. I, The dual luciferase reporter assay determined the interaction between miR‐30a‐3p and 3′UTR of MTHFD2. J, CCK‐8 assay assessed the viability of PC‐9 and H1975 cells after co‐transfected with NC mimics or miR‐30a‐3p mimics, and vector or MTHFD2 plasmid. *P < .05, **P < .01, ***P < .001