The high expression of miR‐144 in blood of patients and mice with TRALI. A, The expression of miR‐144 in blood samples of healthy donors and patients with TRALI, determined by RT‐qPCR. B, The expression of miR‐144 in blood samples of normal mice and TRALI mice evaluated by RT‐qPCR. C, FISH assay to measure miR‐144 level in lung tissues of patients with TRALI (×400). D, The formation of NET in blood samples of healthy donors and patients with TRALI detected by MPO‐DNA ELISA method. E, The formation of NET in blood samples of normal mice and TRALI mice detected by MPO‐DNA ELISA method. F, Flow cytometry to detect the proportion of CD4+ cells in BALF of mice. G, ELISA to measure levels of IL‐6, IL‐8 and IL‐10 in BALF of mice. The measurement data were expressed by the mean ± standard deviation. The comparison between the two groups was analysed by an unpaired t test. The experiment was repeated 3 times independently. * P < .05 vs. healthy donors. # P < .05 vs. normal mice. n = 10