Fig. 1.

Observation on gill activity and body movement of Ozobranchus jantseanus after freezing at −80°C and liquid nitrogen for 96 h. (A) χ²=120.00 (5 min), 120.00 (10 min), 45.52 (30 min); all P<0.01. The gill activity data for resuscitated for 600 min after freezing were analyzed by Fisher's exact tests, P<0.01. (B) χ²=72.00 (10 min), 120.00 (30 min), 120.00 (60 min), 98.18 (120 min); all P<0.01. The data for body movement after resuscitation for 5 min and 600 min after freezing were analyzed by Fisher's exact test, both P<0.01. Values are presented as means±s.e.m. (N=20). Significant differences are indicated by an asterisk (*).