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. 2021 Jun 30;13:645649. doi: 10.3389/fnagi.2021.645649

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Copyright © 2021 Xu, Mei, Lu, Wu, Dong, Shi, Zhou and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune response and chemotaxis term were activated in post-SAH microglia. (A) GO enrichment analysis was performed by Metascape on all DEGs in sham and SAH microglia. Shown are the z-scores of biological functions on inflammatory and immune response-related pathways. (B) Shown are the z-scores of biological functions on cytokine and chemokine-related pathways. (C) Expression profiles of DEGs (fold change > 2, adj_pval < 0.01) in SAH microglia related to leukocyte recruitment pathways. (D–F) Flow cytometric analysis showed that the number of Ly6c+ and Ly6g+ cells increased after SAH, n = 4 per mice group. DEG, differentially expressed gene. *P < 0.05, ***P < 0.001.