FIGURE 1.

Neddylation is activated after MRSA-induced bloodstream infection. Mice were i.v. injected with S. aureus strain USA300 at a dose of 1 × 10⁸ CFUs. Tissues were harvested at 0 (determined as control), 6, 12, and 24 h after USA300 injection (n = 3). (A) Time-dependent changes in relative Nedd8 mRNA expression in the liver, spleen, lung, and kidney tissues after USA300 infection were measured by real-time PCR. (B) NEDD8-Cullins in the liver, spleen, lung, and kidney tissues was observed by Western blot analysis. β-Actin was used as a sample loading control. (C) Quantification of β-actin–normalized NEDD8-Cullins protein levels [from (B)]. Representative results from three independent experiments are shown (mean and SD) (n = 3). ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired Student t test).