Skip to main content
. 2021 Jun 15;206(12):3000–3009. doi: 10.4049/jimmunol.2001407

FIGURE 2.

FIGURE 2.

SARS-CoV-2 Ags induce IFN and NF-κB signaling. (A) Fold change in IFN reporter activity in RAW, THP1, THP1-IRF3−/−, or THP1-TBK1−/− cells treated with control or polymyxin (Pb) at 10 µg/ml or SARS-CoV-2 Ags (E-Trunc, E-Full, S, or N) at 2 µg/ml and Pb at 10 µg/ml for 24 h (n = 2 experiments: 6 biological and 9 technical replicates for RAW with each viral Ag; n = 3 experiments: 9 biological and 6–12 technical replicates for THP1 with each viral Ag; and n = 2 experiments: 9 biological and 9 technical replicates for THP1-IRF3−/−and THP1-TBK1−/−cells with each viral Ag). (B) Fold change in NF-κB reporter activity in THP1 cells treated with control or Pb at 10 µg/ml or SARS-CoV-2 Ags (E-Trunc, E-Full, S, or N) at 2 µg/ml and Pb at 10 µg/ml for 24 h (n = 3 experiments: 9 biological and 6–12 technical replicates for THP1 with each viral Ag). Graphs depict average with SEM. *p < 0.05, ****p < 0.0001. ns, not statistically significant, by Mann–Whitney U test.