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. 2021 Jun 30;12:691835. doi: 10.3389/fpls.2021.691835

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Copyright © 2021 Kong, Sheng, Liang, Ali, Gu, Wu, Chen, Liu and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Melatonin and MT-homologs induce the H₂O₂ accumulation in N. benthamiana leaves. Detection of hydrogen peroxide (H₂O₂) in N. benthamiana leaves treated with various test compounds as determined by diaminobenzidine (DAB) staining. (A) Photographs of representative ethanol-bleached leaves 8 h after treatment with 50 μM solutions of MT, 5-methoxytryptamine, and 5-methoxyindole. (B) Quantitative scoring of staining in treated leaves. Data are the mean ± standard deviations (SDs; n = 27). Different letters indicate that the means are statistically significantly different at p < 0.01.