Figure 4.

Effect of melatonin, 5-methoxytryptamin, and 5-methoxyindoleregulate on the expression of genes involved in H₂O₂ and NO production. The effect of MT and MT-homologs on the relative expression of genes involved in hydrogen peroxide and nitric oxide production. N. benthamiana plants were treated with water (control) or 50 μM solutions of MT, 5-methoxytryptamine, or 5-methoxyindole. The expression level of robhA (A), robhB (B), NIA1 (C), and NIA2 (D) gene in leaves was determined by RT-qPCR. Data are mean ± standard deviation (SDs; n = 9) of three independent experiments. Columns with different letters indicate a significant difference (p ≤ 0.01) between the treatment groups as determined by a Duncan’s multiple range test.