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. 2021 Jun 30;12:691835. doi: 10.3389/fpls.2021.691835

Figure 6.

Figure 6

Bioinformatic prediction of four membrane receptors potentially involved in binding melatonin in N. benthamiana. Bioinformatic prediction and analysis of four putative transmembrane MT-receptor proteins in N. benthamiana. (A) Multiple sequence alignment of the known MT receptor in Arabidopsis (CAND2/PMTR1) with the putative MT-transmembrane receptors, trP47363, trP13076, trP49122, and trP40966 in N. benthamiana. Alignments were carried out using CLUSTAL (http://clustalw.genome.jp). Identical and similar amino acids are indicated by dark and light shading, respectively. (B) Evolutionary relationships between the known MT receptor (CAND2/PMTR1) in Arabidopsis and the putative MT receptors (trP47363, trP13076, trP49122, and trP40966) in N. benthamiana. The evolutionary history was inferred using the Neighbor-Joining method within the MEGA7 program. (C) The predicted transmembrane structure of CAND2 and trP47363, trP13076, trP49122, and trP40966 as determined using HMMTOP software (http://www.sacs.ucsf.edu/cgi-bin/hmmtop.py). The bold, underlined amino acid sequence indicates the putative transmembrane region.