Figure 8.

Melatonin, 5-methoxytryptamin, and 5-methoxyindole induce stomatal closure in N. benthamiana leaves involve the transmembrane receptors trP47363 and trP13076. MT-, 5-methoxytryptamin-, and 5-methoxyindole-induced stomatal closure in leaves of VIGS mutants of N. benthamiana revealing the role of the transmembrane receptors, trP47363 and trP13076. Two-week-old N. benthamiana plants were infiltrated with a mixture of A. tumefaciens Gv3101 strains carrying pTRV1 or pTRV2 constructs as described in the Materials and Methods. Five weeks later, plants were watered with 0.005% (v/v) ethanol/water (control) or 50 μM solutions of MT, 5-methoxytryptamine, or 5-methoxyindole. Stomata were observed under a microscope 3 h after the application of the treatment solutions. (A) Representative low- and high-magnification images. (B) Measurements of stomatal aperture. Data are the mean ± standard deviation (SD; n = 150). Columns with different letters indicate significant differences between the different treatment groups in the different VIGS lines as determined by a Duncan’s multiple range test.