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. 2021 Jun 30;12:689476. doi: 10.3389/fmicb.2021.689476

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Copyright © 2021 Smith and Schuster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Consistency of images captured with the imaging box. (A) Percent brightness of an image standard over a 7-day time course. The dimmer was removed from the apparatus for this series. Dots represent individual replicates (n = 4), and the bars represent means. The first time point is set to 100% brightness. (B) Percent brightness of an image standard captured at different dimmer settings. Dots represent individual replicates (n = 4), and the bars represent means. The brightness value obtained at the maximum dimmer setting is scaled to 100%. (C) Image standard used. The dashed line box in the white rectangle shows the area that was analyzed for mean brightness values in ImageJ.