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. 2021 Jun 30;11:650054. doi: 10.3389/fonc.2021.650054

Figure 2.

Figure 2

PCAT6 promoted NED, proliferation, and invasion of PCa cells in vitro. (A) qPCR analysis of NE markers (NSE, SYP, and ChgA) mRNA expression in LNCaP cells after PCAT6 overexpression. (B, C) Western blot analysis for NSE, SYP, and ChgA protein level in LNCaP cells after PCAT6 overexpression. (D) qPCR analysis of NSE, SYP, and ChgA mRNA expression in NCI-H660 cells after PCAT6 knockdown. (E) LNCaP cell proliferation was assayed using CCK-8 after PCAT6 overexpression. (F) NCI-H660 cell proliferation was assayed using CCK-8 after PCAT6 knockdown. (G, H) LNCaP cell invasion was assessed using transwell invasion assay after PCAT6 overexpression. (I, J) NCI-H660 cell invasion was assessed using transwell invasion assay after PCAT6 knockdown. *p<0.05. **p<0.01.