Skip to main content
. 2021 Jun 30;11:650054. doi: 10.3389/fonc.2021.650054

Figure 5.

Figure 5

PCAT6 activated Hnrnpa2b1 expression in miR-326-dependent manner. (A) Four bioinformatics algorithms (miRanda, miRmap, picTar, and TargetScan) were used to predict the common target genes of miR-326. (B) Luciferase activity was assayed in NCI-H660 cells co-transfected with luciferase reporters containing SRPRA-WT, VLDLR-WT, TNL1-WT, LARP1-WT, GPI-WT, HNRNPA2B1-WT, or BSDC1-WT and miR-326 or miR-326-Mut. (C) Schematic representation of the miR-326 site in Hnrnpa2b1-3’UTR. (D) Luciferase activity was assayed in NCI-H660 cells co-transfected with miR-326 and luciferase reporters containing Hnrnpa2b1-WT or Hnrnpa2b1-Mut. (E) Western blot analysis for Hnrnpa2b1 protein level in NCI-H660 cells after miR-326 overexpression. (F) Luciferase activity was assayed in NCI-H660 cells co-transfected with luciferase reporters containing Hnrnpa2b1-3’ UTR, Hnrnpa2b1-3’ UTR-Mut, PCAT6-WT, or PCAT6-Mut. (G) qPCR analysis of Hnrnpa2b1 mRNA expression in NCI-H660 cells after PCAT6 overexpression in the presence or absence of miR-326. (H, I) Western blot analysis for Hnrnpa2b1 protein level in NCI-H660 cells after PCAT6 overexpression in the presence or absence of miR-326. **p<0.01.