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. 2021 Jun 30;11:650054. doi: 10.3389/fonc.2021.650054

Figure 6.

Figure 6

The role of PCAT6/miR-326/Hnrnpa2b1 axis in regulating PCa cells proliferation, invasion, and NED. (A) qPCR analysis of NSE, SYP, and ChgA mRNA expression in LNCaP cells after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. (B, C) Western blot analysis for NSE, SYP, and ChgA protein level in LNCaP cells after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. (D) LNCaP cell proliferation was assayed using CCK-8 after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. (E, F) LNCaP cell invasion was assessed using transwell invasion assay after PCAT6 overexpression in the presence or absence of miR-326 overexpression or Hnrnpa2b1 knockdown. **p<0.01.