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Collagen-based membranes are biocompatible and allow for adhesion and formation of monolayers of cells. Immunolabeling of cells on collagen-based membranes revealed a continuous distribution of each cell type indicated by DAPI (nuclei) and actin filament staining. These cells were positive for their respective cell–cell adhesion markers: VE-cadherin expression for hiPSC-EC and ZO-1 expression for ARPE-19, inset showing the highlighted area. Scale bars: 50 μm.
