REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-Cleaved Caspase-3 (Asp175)	Cell Signaling	9661; RRID:AB_2341188
anti-phospho-Histone H3 (Ser10)	Millipore	06-570; RRID:AB_310177
anti-GFP	Abcam	ab6673; RRID:AB_305643
anti-Phospho-c-Jun (Ser73) (D47G9)	Cell Signaling	3270; RRID:AB_2129575
anti-Phospho-JNK1+JNK2 (T183 + Y185)	Abcam	ab4821; RRID:AB_2141012
anti-Ly-6A/E (Sca-1)	Biolegend	108101; RRID:AB_313338
Chicken anti-Rat, Alexa Fluor 647	ThermoFisher Scientific	A21472; RRID:AB_2535875
Donkey anti-Goat, Alexa Fluor 555	ThermoFisher Scientific	A32816; RRID:AB_2762839
Chicken anti-Rabbit, Alexa Fluor 647	ThermoFisher Scientific	A21443; RRID:AB_2535861
Donkey anti-Rabbit, Alexa Fluor 568	ThermoFisher Scientific	A10042; RRID:AB_2534017
Donkey anti-Goat, Alexa Fluor 488	ThermoFisher Scientific	A11055; RRID:AB_2534102
Chemicals, peptides, and recombinant proteins
Advanced DMEM F/12	ThermoFisher Scientific	12634-010
B27	ThermoFisher Scientific	17504-044
Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2	R&D Systems	3533-005-02
N-acetylcysteine	Sigma-Aldrich	A9165
TryplE	Thermo Fisher Scientific	12605-010
Dapi	Thermo Fisher Scientific	D1306
Phalloidin Alexa Fluor 647	Thermo Fisher Scientific	A22287
GlutaMAX	Thermo Fisher Scientific	35050-068
HEPES	Thermo Fisher Scientific	15630-056
Penicillin/streptomycin	Thermo Fisher Scientific	15140-122
mEGF	Peprotech	315-09
Noggin	prepared in house	n/a
R-spondin1	prepared in house	n/a
Y-27632	Abmole	M1817
CHIR-99021	Tocris	4423
Valproic acid	Sigma Aldrich	PHR1061-1G
JNK-IN-8	Sigma Aldrich	SML1246
Doxycycline	Sigma Aldrich	D9891
Critical commercial assays
Click-iT EdU Cell Proliferation Kit for Imaging Alexa Fluor 647	ThermoFischer Scientific	C10340
Deposited data
mFetal	(Yui et al., 2018)	N/A
mRepair	(Yui et al., 2018)	N/A
RNA-seq data	NCBI GEO	GEO: GSE176027
Experimental models: cell lines
Villin-CreERT2Apcfl/flKrasG12D/WTTr53fl/R172H	(Fumagalli et al., 2017).	N/A
Villin-CreERT2KrasG12D/WTTrp53fl/flRosa26N1icd/+	ACRCelerate Colorectal Cancer Stratified Medicine Network Consortium (Jackstadt et al., 2019)	N/A
Villin-CreERT2Apcfl/+Trp53fl/flRosa26N1icd/+	ACRCelerate Colorectal Cancer Stratified Medicine Network Consortium (Jackstadt et al., 2019)	N/A
Experimental models: organisms/strains
Mus musculus_Lgr5DTR-eGFP	Genentech (Tian et al., 2011)	N/A
Mus musculus_Gt(ROSA) 26Sortm4(ACTB-tdTomato,-EGFP)Luo/J	The Jackson Laboratory	007676
Mus musculus_eCadherin-mCFP	(Snippert et al., 2010)	N/A
Recombinant DNA
A dual lentiviral vector 3rd generation Tet-regulatory protein expression system for doxycycline-inducible expression of MKP5	VectorBuilder Inc	N/A
pLentiPGK Hygro DEST H2B-mCerulean3	Addgene	90234
pMDLg/pRRE	Addgene	12251
pRSV-Rev	Addgene	12253
pMD2.G	Addgene	12259
Software and algorithms
Imaris	Oxford Instruments	9.3.1
FIJI	https://imagej.net	2.1.0/1.53h
Prism	GraphPad	9.0.0 (86)
ZEN	Zeiss	2.6 (Blue edition)
FlowJo 10.6.1	BD Biosciences	https://www.flowjo.com/
R software	GNU project	https://www.r-project.org/
Other
ibiTreat #1.5 polymer coverslip 96 Well μ-Plate	IBIDI	89626
μ-Slide 8 Well chambered slides	IBIDI	80827
glass-bottom 96 well SensoPlate	Greiner Bio-One	655892