Table 1.

Treatment of Mice with 20S(OH)D3 Modulates Cytokine Production by Lymph node cells.

Treatment of mice	IFNγ	GCSF	GMCSF	IL-17	IL-1α	TNFα
	Pg/ml	Pg/ml	Pg/ml	Pg/ml	Pg/ml	Pg/ml
VEHICLE	6008 ± 174	1859 ± 870	711 ± 277	339 ± 10	33 ± 10	65 ± 13
20S(OH)D3	36 ± 20	50 ± 23	23 ± 19	2 ± 1	4 ± 1	3 ≤ 1
p-Value	0.001	0.001	0.001	0.001	0.001	0.001

Female DBA/1 Lac J mice (N=6 per group) were treated daily after immunization with CII for 14 days with i.p. 20S(OH)D3 (2.5 µg/kg) in 50 µl sesame oil (S.O.) vehicle or with 50 µl of S.O. vehicle daily. Mice were euthanized and lymph node cells were isolated and cultured as described in Methods for 72 h with 1 µg/ml anti CD3/CD28 monoclonal antibodies for 72 h after which the culture supernatants were harvested and analyzed by multiplex cytokine assay and a Luminex instrument. Differences between groups of vehicle-and 20S(OH)D3-treated mice were analyzed by repeated measures ANOVA.