Table 2.
Treatment of CIA mice with 20S(OH)D3 reduces percentages of CD 4+ T cells and B cells in draining lymph nodes while elevating FoxP3 Treg/CD3+ CD4+ ratio*.
| In Vivo | Treatment Percentage | ||||
|---|---|---|---|---|---|
| CD3+ CD4+ T cells | CD4+ CD25+ FoxP3 Tregs | CD19+ B cells | CD4+ CD25 FoxP3+ Tregs/CD3+ CD4+ T cells | ||
| Sesame oil (100 µl/day | 12.1 ± 0.54 | 8.8 ± 1.9 | 17.7 ± 3.5 | 1.1 ± 0.11 | |
| 20S(OH)D3 (2.4 µg/kg/day | 9.1 ± 0.5 | 7.0 ± 1.04 | 11.4 ± 0.7 | 1.6 ± 0.14 | |
| pValue | 0.004 | NS | 0.004 | 0.023 | |
*Lymph nodes were harvested from DB A/1 LacJ mice immunized with CII and treated daily for 14 days with i.p. S.O. (n=5) or 20S(OH)D3 (2.4 µg/kg) (n=5) dissolved in S.O. Percentage of CD3+ T cells and B cells were determined by flow cytometry after staining with fluorochrome tagged rat anti-mouse CD3, anti-mouse CD19. FoxP3 Tregs were detected by FoxP3 staining kit (eBioscience, San Diego, CA).
NS, not significant.