Table 2.
Optimized multiple reaction monitoring transition and parameters for 20 amino acids and the internal standard (IS).
| Molecular weight | Precursor ion (m/z) | Product ion (m/z) | Declustering potential (volts) | Collision energy (volts) | Collision cell exit potential (volts) | Curtain gas (psi) | Collision activated dissociation gas (psi) | Ion source gas1 (psi) | Ion source gas2 (psi) | |
|---|---|---|---|---|---|---|---|---|---|---|
| Alanine | 89.1 | 90.0 | 44.0 | 56 | 17 | 12 | 40 | 8 | 30 | 40 |
| Arginine | 174.2 | 175.0 | 69.9 | 51 | 29 | 10 | 40 | 8 | 30 | 40 |
| Asparagine | 132.1 | 132.9 | 74.1 | 31 | 21 | 12 | 40 | 8 | 30 | 40 |
| Aspartic acid | 133.1 | 133.9 | 74.0 | 61 | 19 | 8 | 40 | 8 | 30 | 40 |
| Cysteine | 121.2 | 122.0 | 59.0 | 31 | 35 | 10 | 40 | 8 | 30 | 40 |
| Glutamic acid | 147.1 | 147.9 | 83.9 | 36 | 23 | 14 | 40 | 8 | 30 | 40 |
| Glutamine | 146.2 | 147.0 | 84.0 | 56 | 23 | 10 | 30 | 10 | 50 | 80 |
| Glycine | 75.1 | 75.9 | 30.1 | 56 | 17 | 14 | 30 | 10 | 50 | 80 |
| Histidine | 155.2 | 156.0 | 110.0 | 31 | 19 | 10 | 40 | 8 | 30 | 40 |
| Isoleucine | 131.2 | 132.0 | 86.1 | 51 | 15 | 12 | 40 | 8 | 30 | 40 |
| Leucine | 131.2 | 132.0 | 86.0 | 46 | 15 | 8 | 40 | 8 | 30 | 40 |
| Lysine | 146.2 | 147.0 | 84.0 | 31 | 25 | 14 | 30 | 10 | 50 | 80 |
| Methionine | 149.2 | 150.0 | 104.0 | 36 | 15 | 14 | 40 | 8 | 30 | 40 |
| Phenylalanine | 165.2 | 166.0 | 102.9 | 46 | 37 | 16 | 40 | 8 | 30 | 40 |
| Proline | 115.1 | 116.1 | 70.0 | 61 | 21 | 10 | 30 | 10 | 50 | 80 |
| Serine | 105.1 | 105.9 | 59.9 | 81 | 15 | 12 | 40 | 8 | 30 | 40 |
| Threonine | 119.1 | 119.8 | 74.0 | 41 | 15 | 10 | 40 | 8 | 30 | 40 |
| Tryptophan | 204.2 | 205.0 | 187.9 | 76 | 15 | 18 | 40 | 8 | 30 | 40 |
| Tyrosine | 181.2 | 182.0 | 136.0 | 36 | 19 | 16 | 40 | 8 | 30 | 40 |
| Valine | 117.2 | 118.0 | 72.0 | 41 | 15 | 8 | 40 | 8 | 30 | 40 |
| Alanine-2,3,3,3-d4 (IS) | 93.1 | 94.0 | 48.1 | 111 | 15 | 8 | 40 | 8 | 30 | 40 |