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. Author manuscript; available in PMC: 2021 Jul 14.
Published in final edited form as: Nature. 2020 Jun 24;584(7819):98–101. doi: 10.1038/s41586-020-2428-0

Extended data Figure 2: Lowering the pHi can reversibly slow down embryo elongation.

Extended data Figure 2:

(a-b) Snapshots of 2-day chicken embryos cultured in minimal medium at pH 7.2 with 8.3 mM (n=6) glucose or 0.83 mM glucose (n=3).

(c-d) Snapshots of 2-day chicken embryos cultured in minimal medium with 8.3 mM glucose in acidic conditions (pH6.0: n=6, pH5.3: n=6)

(e) Snapshots of a 2-day chicken embryo first cultured in minimal medium with 8.3 mM glucose at pH5.3 showing the arrest of elongation after 9 hours and returned to control medium after 10.5 hours showing the rescue of elongation (n=6).

Bright field micrographs of the posterior region of chicken embryos taken at 1.5 hour intervals. Somites formed at the last time point are indicated by asterisks on the right. Ventral views, anterior to the top. Scale bar: 100 μm.