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. Author manuscript; available in PMC: 2021 Jul 14.
Published in final edited form as: Nature. 2020 Jun 24;584(7819):98–101. doi: 10.1038/s41586-020-2428-0

Extended data Figure 4: kinetics of Wnt/β-catenin signaling during human iPS differentiation to PSM.

Extended data Figure 4:

(a) Immunohistochemistry showing the dynamic expression of β-catenin and Venus (YFP) proteins in human MSGN1-Venus iPS reporter cells differentiated to the PSM fate in vitro (n=3). Hoechst labeling of the nuclei is shown in blue. Scale bar: 30 μm

(b) Quantification of the intensity of nuclear localization of β-catenin shown in (a) using Fiji. Mean +/− SD, (Day 0, D1, D4, n=3; D2, D3, n=4). One-way ANOVA followed by Tukey’s multiple comparisons test: Day1 versus Day2, P=0.0411, Day2 versus Day3, P=0.0038, Day2 versus Day4, P=0.0009. *p < 0.05; **p< 0.01; ***p< 0.001.

(c-e) qPCR analysis comparing the expression level of MSGN1 (c), TBX6 (d) and AXIN2 (e) of human iPS cells differentiating to the PSM fate in vitro. Values were normalized by the results of differentiation at day 0. Mean +/− SD (n=3). One-way ANOVA followed by Tukey’s multiple comparisons test: TBX6; Day0 versus Day2, P<0.0001, Day0 versus Day3, P<0.0001, Day3 versus Day4, P<0.0001, Day4 versus Day10, P=0.0466, AXIN2; Day0 versus Day1, P=0.0006, Day0 versus Day3, P<0.0001, Day3 versus Day4, P=0.0003. *p < 0.05; ***p< 0.001; ****p< 0.0001.