Extended data Figure 6: β-catenin acetylation depends on intracellular pH and glycolytic activity.

(a-b) Western blot analysis using anti-acetylated K49 β-catenin, anti-active β-catenin, anti-actin and anti-β-catenin antibodies. Extracts of 2-day chicken embryos cultured in minimal medium at pH7.2 with various glucose concentrations (n=4 per condition) (a) or in minimal medium with 8.3 mM glucose at various pH (n=3 per condition) (b).

(c) Quantification of acetylated lysine and β-catenin intensity in Fig. 3h using Fiji. Acetylation rate is calculated from the slope of graph. n=2 independent experiments. Linear approximation, mean +/− SD of slope.

For gel source data, see Supplementary Figure 1